Role of disulfide bond isomerase DsbC, calcium ions, and hemin in cell-free protein synthesis of active manganese peroxidase isolated from Phanerochaete chrysosporium.
Identifieur interne : 000301 ( Main/Exploration ); précédent : 000300; suivant : 000302Role of disulfide bond isomerase DsbC, calcium ions, and hemin in cell-free protein synthesis of active manganese peroxidase isolated from Phanerochaete chrysosporium.
Auteurs : Ryoko Ninomiya [Japon] ; Bo Zhu [Japon] ; Takaaki Kojima [Japon] ; Yugo Iwasaki [Japon] ; Hideo Nakano [Japon]Source :
- Journal of bioscience and bioengineering [ 1347-4421 ] ; 2014.
Descripteurs français
- KwdFr :
- Biosynthèse des protéines (effets des médicaments et des substances chimiques), Calcium (métabolisme), Calcium (pharmacologie), Escherichia coli (métabolisme), Hémagglutinines (métabolisme), Hémine (métabolisme), Hémine (pharmacologie), Modèles moléculaires (MeSH), Peroxidases (biosynthèse), Peroxidases (isolement et purification), Peroxidases (métabolisme), Phanerochaete (enzymologie), Phanerochaete (génétique), Pliage des protéines (effets des médicaments et des substances chimiques), Protein Disulfide-Isomerases (métabolisme), Solubilité (MeSH), Système acellulaire (effets des médicaments et des substances chimiques), Tests de criblage à haut débit (MeSH), Transcription génétique (effets des médicaments et des substances chimiques), Évolution moléculaire dirigée (MeSH).
- MESH :
- biosynthèse : Peroxidases.
- effets des médicaments et des substances chimiques : Biosynthèse des protéines, Pliage des protéines, Système acellulaire, Transcription génétique.
- enzymologie : Phanerochaete.
- génétique : Phanerochaete.
- isolement et purification : Peroxidases.
- métabolisme : Calcium, Escherichia coli, Hémagglutinines, Hémine, Peroxidases, Protein Disulfide-Isomerases.
- pharmacologie : Calcium, Hémine.
- Modèles moléculaires, Solubilité, Tests de criblage à haut débit, Évolution moléculaire dirigée.
English descriptors
- KwdEn :
- Calcium (metabolism), Calcium (pharmacology), Cell-Free System (drug effects), Directed Molecular Evolution (MeSH), Escherichia coli (metabolism), Hemagglutinins (metabolism), Hemin (metabolism), Hemin (pharmacology), High-Throughput Screening Assays (MeSH), Models, Molecular (MeSH), Peroxidases (biosynthesis), Peroxidases (isolation & purification), Peroxidases (metabolism), Phanerochaete (enzymology), Phanerochaete (genetics), Protein Biosynthesis (drug effects), Protein Disulfide-Isomerases (metabolism), Protein Folding (drug effects), Solubility (MeSH), Transcription, Genetic (drug effects).
- MESH :
- chemical , biosynthesis : Peroxidases.
- chemical , isolation & purification : Peroxidases.
- chemical , metabolism : Calcium, Hemagglutinins, Hemin, Peroxidases, Protein Disulfide-Isomerases.
- chemical , pharmacology : Calcium, Hemin.
- drug effects : Cell-Free System, Protein Biosynthesis, Protein Folding, Transcription, Genetic.
- enzymology : Phanerochaete.
- genetics : Phanerochaete.
- metabolism : Escherichia coli.
- Directed Molecular Evolution, High-Throughput Screening Assays, Models, Molecular, Solubility.
Abstract
A cell-free protein synthesis system can produce various types of proteins directly from DNA templates such as PCR products, and therefore attracts great attention as an alternative protein synthesis system especially for high-throughput functional screening of proteins. Here, we report successful expression of active Phanerochaete chrysosporium manganese peroxidase (MnP) in an Escherichia coli cell-free protein synthesis system, wherein reaction conditions such as the concentrations of hemin, calcium ions, and disulfide bond isomerase were optimized to increase the solubility and activity of the synthesized enzyme. Moreover, cell-free synthesized MnP purified using the hemagglutinin tag showed higher specific activity than the commercial wild-type enzyme, suggesting that the cell-free system can be used as a preparative method for efficient synthesis of disulfide bond-containing metalloenzymes such as MnP. We believe that our system is a solid foundation for the development of a high-throughput screening method for the directed evolution of these enzymes.
DOI: 10.1016/j.jbiosc.2013.11.003
PubMed: 24332478
Affiliations:
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Le document en format XML
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Electronic address: hnakano@agr.nagoya-u.ac.jp.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601</wicri:regionArea><wicri:noRegion>Nagoya 464-8601</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of bioscience and bioengineering</title><idno type="eISSN">1347-4421</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Calcium (metabolism)</term><term>Calcium (pharmacology)</term><term>Cell-Free System (drug effects)</term><term>Directed Molecular Evolution (MeSH)</term><term>Escherichia coli (metabolism)</term><term>Hemagglutinins (metabolism)</term><term>Hemin (metabolism)</term><term>Hemin (pharmacology)</term><term>High-Throughput Screening Assays (MeSH)</term><term>Models, Molecular (MeSH)</term><term>Peroxidases (biosynthesis)</term><term>Peroxidases (isolation & purification)</term><term>Peroxidases (metabolism)</term><term>Phanerochaete (enzymology)</term><term>Phanerochaete (genetics)</term><term>Protein Biosynthesis (drug effects)</term><term>Protein Disulfide-Isomerases (metabolism)</term><term>Protein Folding (drug effects)</term><term>Solubility (MeSH)</term><term>Transcription, Genetic (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Biosynthèse des protéines (effets des médicaments et des substances chimiques)</term><term>Calcium (métabolisme)</term><term>Calcium (pharmacologie)</term><term>Escherichia coli (métabolisme)</term><term>Hémagglutinines (métabolisme)</term><term>Hémine (métabolisme)</term><term>Hémine (pharmacologie)</term><term>Modèles moléculaires (MeSH)</term><term>Peroxidases (biosynthèse)</term><term>Peroxidases (isolement et purification)</term><term>Peroxidases (métabolisme)</term><term>Phanerochaete (enzymologie)</term><term>Phanerochaete (génétique)</term><term>Pliage des protéines (effets des médicaments et des substances chimiques)</term><term>Protein Disulfide-Isomerases (métabolisme)</term><term>Solubilité (MeSH)</term><term>Système acellulaire (effets des médicaments et des substances chimiques)</term><term>Tests de criblage à haut débit (MeSH)</term><term>Transcription génétique (effets des médicaments et des substances chimiques)</term><term>Évolution moléculaire dirigée (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Peroxidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Peroxidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Calcium</term><term>Hemagglutinins</term><term>Hemin</term><term>Peroxidases</term><term>Protein Disulfide-Isomerases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Calcium</term><term>Hemin</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Peroxidases</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell-Free System</term><term>Protein Biosynthesis</term><term>Protein Folding</term><term>Transcription, Genetic</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Biosynthèse des protéines</term><term>Pliage des protéines</term><term>Système acellulaire</term><term>Transcription génétique</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Peroxidases</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Calcium</term><term>Escherichia coli</term><term>Hémagglutinines</term><term>Hémine</term><term>Peroxidases</term><term>Protein Disulfide-Isomerases</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Calcium</term><term>Hémine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Directed Molecular Evolution</term><term>High-Throughput Screening Assays</term><term>Models, Molecular</term><term>Solubility</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Modèles moléculaires</term><term>Solubilité</term><term>Tests de criblage à haut débit</term><term>Évolution moléculaire dirigée</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A cell-free protein synthesis system can produce various types of proteins directly from DNA templates such as PCR products, and therefore attracts great attention as an alternative protein synthesis system especially for high-throughput functional screening of proteins. Here, we report successful expression of active Phanerochaete chrysosporium manganese peroxidase (MnP) in an Escherichia coli cell-free protein synthesis system, wherein reaction conditions such as the concentrations of hemin, calcium ions, and disulfide bond isomerase were optimized to increase the solubility and activity of the synthesized enzyme. Moreover, cell-free synthesized MnP purified using the hemagglutinin tag showed higher specific activity than the commercial wild-type enzyme, suggesting that the cell-free system can be used as a preparative method for efficient synthesis of disulfide bond-containing metalloenzymes such as MnP. We believe that our system is a solid foundation for the development of a high-throughput screening method for the directed evolution of these enzymes. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">24332478</PMID><DateCompleted><Year>2014</Year><Month>08</Month><Day>19</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1347-4421</ISSN><JournalIssue CitedMedium="Internet"><Volume>117</Volume><Issue>5</Issue><PubDate><Year>2014</Year><Month>May</Month></PubDate></JournalIssue><Title>Journal of bioscience and bioengineering</Title><ISOAbbreviation>J Biosci Bioeng</ISOAbbreviation></Journal><ArticleTitle>Role of disulfide bond isomerase DsbC, calcium ions, and hemin in cell-free protein synthesis of active manganese peroxidase isolated from Phanerochaete chrysosporium.</ArticleTitle><Pagination><MedlinePgn>652-7</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jbiosc.2013.11.003</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S1389-1723(13)00406-4</ELocationID><Abstract><AbstractText>A cell-free protein synthesis system can produce various types of proteins directly from DNA templates such as PCR products, and therefore attracts great attention as an alternative protein synthesis system especially for high-throughput functional screening of proteins. Here, we report successful expression of active Phanerochaete chrysosporium manganese peroxidase (MnP) in an Escherichia coli cell-free protein synthesis system, wherein reaction conditions such as the concentrations of hemin, calcium ions, and disulfide bond isomerase were optimized to increase the solubility and activity of the synthesized enzyme. Moreover, cell-free synthesized MnP purified using the hemagglutinin tag showed higher specific activity than the commercial wild-type enzyme, suggesting that the cell-free system can be used as a preparative method for efficient synthesis of disulfide bond-containing metalloenzymes such as MnP. We believe that our system is a solid foundation for the development of a high-throughput screening method for the directed evolution of these enzymes. </AbstractText><CopyrightInformation>Copyright © 2013 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ninomiya</LastName><ForeName>Ryoko</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Bo</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kojima</LastName><ForeName>Takaaki</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Iwasaki</LastName><ForeName>Yugo</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nakano</LastName><ForeName>Hideo</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan. Electronic address: hnakano@agr.nagoya-u.ac.jp.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>12</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>J Biosci Bioeng</MedlineTA><NlmUniqueID>100888800</NlmUniqueID><ISSNLinking>1347-4421</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006388">Hemagglutinins</NameOfSubstance></Chemical><Chemical><RegistryNumber>743LRP9S7N</RegistryNumber><NameOfSubstance UI="D006427">Hemin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="D010544">Peroxidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.13</RegistryNumber><NameOfSubstance UI="C051129">manganese peroxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 5.3.4.1</RegistryNumber><NameOfSubstance UI="D019704">Protein Disulfide-Isomerases</NameOfSubstance></Chemical><Chemical><RegistryNumber>SY7Q814VUP</RegistryNumber><NameOfSubstance UI="D002118">Calcium</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002118" MajorTopicYN="N">Calcium</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002474" MajorTopicYN="Y">Cell-Free System</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019020" MajorTopicYN="N">Directed Molecular Evolution</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006388" MajorTopicYN="N">Hemagglutinins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006427" MajorTopicYN="N">Hemin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D057166" MajorTopicYN="N">High-Throughput Screening Assays</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008958" MajorTopicYN="N">Models, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010544" MajorTopicYN="N">Peroxidases</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014176" MajorTopicYN="N">Protein Biosynthesis</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019704" MajorTopicYN="N">Protein Disulfide-Isomerases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017510" MajorTopicYN="N">Protein Folding</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012995" MajorTopicYN="N">Solubility</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Disulfide bond isomerase</Keyword><Keyword MajorTopicYN="N">In vitro transcription/translation</Keyword><Keyword MajorTopicYN="N">Manganese peroxidase</Keyword><Keyword MajorTopicYN="N">Metalloenzyme</Keyword><Keyword MajorTopicYN="N">Protein folding</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>07</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2013</Year><Month>10</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>11</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>12</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>12</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>8</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24332478</ArticleId><ArticleId IdType="pii">S1389-1723(13)00406-4</ArticleId><ArticleId IdType="doi">10.1016/j.jbiosc.2013.11.003</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Ninomiya, Ryoko" sort="Ninomiya, Ryoko" uniqKey="Ninomiya R" first="Ryoko" last="Ninomiya">Ryoko Ninomiya</name></noRegion><name sortKey="Iwasaki, Yugo" sort="Iwasaki, Yugo" uniqKey="Iwasaki Y" first="Yugo" last="Iwasaki">Yugo Iwasaki</name><name sortKey="Kojima, Takaaki" sort="Kojima, Takaaki" uniqKey="Kojima T" first="Takaaki" last="Kojima">Takaaki Kojima</name><name sortKey="Nakano, Hideo" sort="Nakano, Hideo" uniqKey="Nakano H" first="Hideo" last="Nakano">Hideo Nakano</name><name sortKey="Zhu, Bo" sort="Zhu, Bo" uniqKey="Zhu B" first="Bo" last="Zhu">Bo Zhu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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